![]() Concentrations as low as 50 mg kg−1 (fresh weight) could be detected. Staining of Ni dissolved in agar at various concentrations was used to calibrate the method. The distribution of Ni could be determined at the cellular level and consistent patterns were obtained for replicates. A Karhunen–Loeve transformation was applied to the images to minimize interference from colours of other origin, e.g. Plant tissue cross-sections were imaged under a microscope immediately after DMG application. The best solution was DMG (10 g l−1) dissolved in borax (25 mM) and KOH (30 mM). We tested a suite of staining methods consisting of dimethylglyoxime (DMG) dissolved in a range of solvents for the mapping of Ni distribution in the Ni hyperaccumulator Berkheya coddii Roessler. Commonly used methods for measuring Ni distribution in plant tissues require expensive equipment and complex sample preparation. Determination of the nickel (Ni) distribution in tissues of hyperaccumulator plants aids in understanding the strategies and mechanisms used by these plants to take up Ni from soils.
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